Looking for (Otter) Parasites
The back of my neck felt as though it was burning to a crisp in the afternoon sun. I took a break from the microscope and stood up long enough to pull up my shirt collar, stretch my legs, and glance at the sky. The sun had begun to sink on the horizon. Soon the mosquitoes would be on the attack, and we still had to make the drive back to Karanambu. Or maybe it would be a boat ride. I wasn’t sure.

Lucy working at the microscope while Stefy prepares samples, at Caiman House Research Station, Yupukari, Guyana
I called over to Stefy (Dr. Stephanie Zanet) to ask her how many samples we had left to read. “Just the repeat exam on Bel and Bandit,” she answered.
Though this was only Stefy’s fifth day in the Rupununi, she seemed completely at ease. She had everything organized. On a table nearby, she’d set out a row of plastic bags of otter poop, each labeled with the patient’s name, a box of clean slides, a cardboard box punched with holes that served as a test tube rack, and a container of sugar water used to run the test known as a “fecal float.”

Our parasitology lab at Yupukari
We’d set up our makeshift lab at Caiman House, in the Makushi village of Yupukari, having traveled there specifically to use their spare microscope. Fortunately, we arrived in time to find the malaria technician at his desk and he willingly loaned us his special equipment. I was ecstatic. For the first time ever in the Rupununi., I found myself looking through a microscope Never mind that it wasn’t electric. We had plenty of sunshine to aim at its old-fashioned mirror.
But the light wouldn’t last forever. I sat back down and peered once again at the slide. Seconds later, I found our first parasitic egg. It was in the sample from Buddy.
“Wow! ” I exclaimed.

Stefy (Dr. Stefy Zanet) preparing samples for fecal analysis
Stefy ran over to take a look. This was our first positive finding, and, in a paradoxical sort of way, we were both excited. It meant our crude methodology had worked, at least to the extent that we could identify a parasitic egg in a two-day- old sample collected from an orphaned giant otter, using the late-afternoon Rupununi sunshine to light up an old-fashioned mirror microscope.
From that moment on, I had no doubt that Stefy would do just fine during her two-and-a-half-month stay as a visiting veterinarian at Karanambu. She’s volunteering her time, and, as I see it, her visit is step in the direction of a one-health program for the ranch. My guess is that she’ll find plenty of eggs as she examines more samples.

Buddy at the Karanambu landing amidst the daily laundry routine
The orphaned otters at Karanambu cross paths all the time with both dogs and people. We know that each species is likely to have its own set of parasites; even so, the first step is to confirm that this is indeed the case. The next is to determine which ones are moving across species lines. If we find that parasites are being passed from one to the other, there’s a good chance that a re-introduced orphaned otter could pick up a new parasite and introduce it to the wild otters. We can—and do—deworm the rehabilitated otters, but this was the first time I’d had a chance to document the reason for doing so.

Orphan otters Bel and Philip in their pen at Karanambu
The challenge has always been the logistics of obtaining and running the samples. During Stefy’s upcoming stay at Karanambu, there will be at least three orphaned otters, a crab-eating raccoon, five domestic dogs, three cats, and a dozen people—perfect timing! We should soon have some answers.

A strongyle-type egg in the fecal sample from giant otter Buddy
Hoping to document the first egg, Stefy grabbed her digital camera. This technique works surprisingly well, as you can see from the photo above. But unfortunately, the light in the microscope’s ield of view wasn’t bright enough. The angle of the sun had changed enough to throw everything out o=of whack. I struggled to brighten the image, but my options were limited. I moved the mirror, then my body, head, and neck, and, eventually, the whole table, trying to capture just the right amount of light. When I got too much, the whole field went black. Meanwhile, my sample was drying rapidly in the hot sun.
Thinking quickly, Stefy produced a tiny flashlight, reminding me that she’d had to do this in Rwanda when the power supply burned out. She was still in vet school then, visiting the mountain gorilla project from Italy for a senior-year internship. I found her a great help with the orphaned gorillas, and when I heard she was looking for more wildlife experience, I suggested she give Karanambu a try.
I’m sure she’ll be blogging about her experiences on Saving Otters very soon—and quite possibly in Italian as well as English.
After our parasitology session, we returned to Karanambu by boat. Though we didn’t see any otters, we passed by several of their dens, or holts.

A holt in use by wild giant otters, Rupununi River, near Yupukari
As usual, we saw plenty of wild caiman. In my next post I’ll write about going out out night “caimaning” (a new verb) with the experts at Caiman House. I’ve put their website up on the blogroll.

Two caiman alligators, seen on our way back to Karanambu by boat from Yupukari





































































Jan 19th Anna C USD 11.00